Search

SKILLS

Immunology
  • Primary cell isolation and culture (lymphocyte, macrophage)
  • Cell-cycle analysis
  • Mitogen enrichment
  • Cell proliferation assay
  • Immunization of murine models
  • Phagocytosis and related assays
  • Enzyme Linked Immunosorbent Assay (ELISA), Enzyme Immuno Assay (EIA)
  • Plaque assay and antibody titre
  • Flow cytometry
  • Inflammatory marker study
  • Cytotoxicity assays
Molecular Biology
  • Extraction and quantification of nucleic acids
  • Polymerase Chain Reaction (PCR), qRT-PCR
  • Gel Electrophoresis
  • ​Western Blotting
Microbiology
  • Bacteria and fungal isolation from various sources
  • Bacterial culture and growth monitoring
  • Transfection
  • Staining techniques
  • Aseptic techniques
Genomics
  • ​16S Illmina data analysis
  • QIIME (microbial abundance, diversity)
  • PICRUST (microbial function)​
Statistics and Computer programs
  • Basic parametric, non-parametric and descriptive statistics
  • Multivariate statistics (PCA, HCA, proximity heat-map)
  • SPSS, KyPlot, Prism, R, etc.
  • Microsoft office package
  • Web designing
Pharmacology and natural product chemistry
  • Extract preparation and fractionation
  • Thin Layer chromatography (TLC), preparative-TLC, High performance-TLC (HPTLC)
  • High Performance Liquid Chromatography (HPLC), Gas Chromatography-Mass Spectrometry (GC-MS), Flame Ionization Detection (FID), Solid-Phase Microextraction (SPME)
  • Mass fragmentation analysis
  • Fourier Transform Infrared Spectroscopy (FTIR)
  • Qualitative and quantitative study of metabolites
  • Pharmacognostic modeling
Microscopy
  • Scanning Electron Microscopy
  • Transmission Electron Microscopy
  • Immuno-electron microscopy
  • Fluorescence Microscopy
Virology
  • Virus infection and growth,
  • Virus purification
  • Determination of virus titres by plaque assay and EIA assay
  • Monitoring virus growth by protein detection by western blotting​ and RT-PCR
  • Transfection of cloned viral genes and detection of protein expression by fluorescence and western blot detection
Additional skills
  • Experienced in working with live laboratory animal models.
  • Animal maintenance and breeding
  • Histopathological analysis
  • Grant/research proposal writing
  • Laboratory maintenance
  • Team player as well as independent worker
  • Experimental designing
  • Fast picking up capabilities
© Priyankar Dey, May, 2023